19 Publications

Aza P, Molpeceres G, de Salas F, Camarero S

[2021] Design of an improved universal signal peptide based on the α-factor mating secretion signal for enzyme production in yeast
Cell Mol. Life Sci., 10.1007/s00018-021-03793-y
Saccharomyces cerevisiae plays an important role in the heterologous expression of an array of proteins due to its easy manipulation, low requirements and ability for protein post-translational modifications. The implementation of the preproleader secretion signal of the [...]

Aza P, de Salas F, Molpeceres G, Rodríguez-Escribano D, de la Fuente I, Camarero S

[2021] Protein Engineering Approaches to Enhance Fungal Laccase Production in S. cerevisiae
Int. J. Mol. Sci., 22: 1157
Laccases secreted by saprotrophic basidiomycete fungi are versatile biocatalysts able to oxidize a wide range of aromatic compounds using oxygen as the sole requirement. Saccharomyces cerevisiae is a preferred host for engineering fungal laccases. To assist the difficult [...]

Talens-Perales D, Sánchez-Torres P, Marín-Navarro J, Polaina J

[2020] In silico screening and experimental analysis of family GH11 xylanases for applications under conditions of alkaline pH and high temperature
Biotechnol. Biofuels, 13: 198
Background Xylanases are one of the most extensively used enzymes for biomass digestion. However, in many instances, their use is limited by poor performance under the conditions of pH and temperature required by the industry. Therefore, the search for xylanases able to [...]

de Salas F, Aza P, Gilabert JF, Santiago G, Kılıç S, Sener ME, Vind J, Guallar V, Martínez AT, Camarero S

[2019] Engineering of a fungal laccase to develop a robust, versatile and highly-expressed biocatalyst for sustainable chemistry
Green Chemistry, 10.1039/c9gc02475a
Fungal laccases can play an important role as biocatalysts in organic chemistry to replace chemical synthesis. In a previous work we synthesized conductive polyaniline using a high-redox potential laccase from our collection of recombinant fungal variants. Still, the oxidation [...]

de Salas F, Cañadas R, Santiago G, Virseda-Jerez A, Vind J, Gentili P, Martínez AT, Guallar V, Muñoz IG, Camarero S

[2019] Structural and biochemical insights into an engineered high-redox potential laccase overproduced in Aspergillus
Int. J. Biol. Macromol., 10.1016/j.ijbiomac.2019.09.052
Fungal laccases have great potential as biocatalysts oxidizing a variety of aromatic compounds using oxygen as co-substrate. Here, the crystal structure of 7D5 laccase (PDB 6H5Y), developed in Saccharomyces cerevisiae and overproduced in Aspergillus oryzae, is compared with [...]

Pardo I, Rodríguez-Escribano D, Aza P, de Salas F, Martínez AT, Camarero S

[2018] A highly stable laccase obtained by swapping the second cupredoxin domain
Scientific Reports, 8: 15669
The robustness of a high-redox potential laccase has been enhanced by swapping its second cupredoxin domain with that from another fungal laccase, which introduced a pool of neutral mutations in the protein sequence without affecting enzyme functionality. The new laccase [...]

Ayuso-Fernández I, Ruiz-Dueñas FJ, Martínez AT

[2018] Evolutionary convergence in lignin-degrading enzymes
Proc. Natl. Acad. Sci. USA, 115: 6428-6433
We analyze the molecular mechanisms that led to the rise of a powerful strategy for lignin degradation (i.e., the formation of a solvent-exposed tryptophanyl radical capable of oxidizing the bulky lignin polymer) as a convergent trait in different species of fungi (order [...]

Martínez AT, Camarero S, Ruiz-Dueñas FJ, Martínez MJ

[2018] Biological Lignin Degradation
Lignin Valorization: Emerging Approaches. Ed. Gregg Beckham. RSC: 199-225
In nature, white-rot fungi and some bacteria secrete powerful oxidative enzymes such as peroxidases and laccases to break down lignin and lignin products. These enzymes, together with different chemical mediators, form a chemo-enzymatic system for delignifying biomass so [...]

Serrano L, Esakkimuthu SE, Marlin N, Brochier-Salon MC, Mortha G, Bertaud F

[2018] Fast, Easy, and Economical Quantification of Lignin Phenolic Hydroxyl Groups: Comparison with Classical Techniques
Energy&Fuels, 32: 5969-5977
The quantification of lignin functional groups constitutes an essential step for effective assessment of lignin transformation. A new, fast, economical, and simple method without the use of harmful solvents is presented for lignin phenolic hydroxyl group quantification. [...]

Hämaläinen V, Grönroos T, Suonpää A, Heikkilä MW, Romein B, Ihalainen P, Malandra S, Birikh KR

[2018] Enzymatic process to unlock the lignin value
Front. Bioeng. Biotechnol., 10.3389/fbioe.2018.00020
Main hurdles of lignin valorization are its diverse chemical composition, recalcitrance, and poor solubility due to high-molecular weight and branched structure. Controlled fragmentation of lignin could lead to its use in higher value products such as binders, coatings, [...]